Review



solubilization stop solution  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Promega solubilization stop solution
    Solubilization Stop Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solubilization stop solution/product/Promega
    Average 90 stars, based on 1 article reviews
    solubilization stop solution - by Bioz Stars, 2026-05
    90/100 stars

    Images



    Similar Products

    solubilization stop solution  (Promega)
    90
    Promega solubilization stop solution
    Solubilization Stop Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solubilization stop solution/product/Promega
    Average 90 stars, based on 1 article reviews
    solubilization stop solution - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    solubilization solution/stop mix  (Promega)
    90
    Promega solubilization solution/stop mix
    Solubilization Solution/Stop Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solubilization solution/stop mix/product/Promega
    Average 90 stars, based on 1 article reviews
    solubilization solution/stop mix - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    solubilization solution/stop mix g401a  (Promega)
    90
    Promega solubilization solution/stop mix g401a
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    Solubilization Solution/Stop Mix G401a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solubilization solution/stop mix g401a/product/Promega
    Average 90 stars, based on 1 article reviews
    solubilization solution/stop mix g401a - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    mtt assay (promega, g4000, including solubilization solution/stop mix g401a and dye solution g402a)  (Promega)
    90
    Promega mtt assay (promega, g4000, including solubilization solution/stop mix g401a and dye solution g402a)
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    Mtt Assay (Promega, G4000, Including Solubilization Solution/Stop Mix G401a And Dye Solution G402a), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtt assay (promega, g4000, including solubilization solution/stop mix g401a and dye solution g402a)/product/Promega
    Average 90 stars, based on 1 article reviews
    mtt assay (promega, g4000, including solubilization solution/stop mix g401a and dye solution g402a) - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    stop solubilization solution  (Promega)
    90
    Promega stop solubilization solution
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    Stop Solubilization Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stop solubilization solution/product/Promega
    Average 90 stars, based on 1 article reviews
    stop solubilization solution - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    solubilization solution/stop mix g4101  (Promega)
    90
    Promega solubilization solution/stop mix g4101
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    Solubilization Solution/Stop Mix G4101, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solubilization solution/stop mix g4101/product/Promega
    Average 90 stars, based on 1 article reviews
    solubilization solution/stop mix g4101 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    solubilization solution/stop mix promega g4101  (Promega)
    90
    Promega solubilization solution/stop mix promega g4101
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    Solubilization Solution/Stop Mix Promega G4101, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solubilization solution/stop mix promega g4101/product/Promega
    Average 90 stars, based on 1 article reviews
    solubilization solution/stop mix promega g4101 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    dmso, the solubilization/stop solution  (Tecan Systems)
    90
    Tecan Systems dmso, the solubilization/stop solution
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    Dmso, The Solubilization/Stop Solution, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmso, the solubilization/stop solution/product/Tecan Systems
    Average 90 stars, based on 1 article reviews
    dmso, the solubilization/stop solution - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    1 ml of solubilization solution/stop mix ®  (Promega)
    90
    Promega 1 ml of solubilization solution/stop mix ®
    <t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
    1 Ml Of Solubilization Solution/Stop Mix ®, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 ml of solubilization solution/stop mix ®/product/Promega
    Average 90 stars, based on 1 article reviews
    1 ml of solubilization solution/stop mix ® - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    TNF-α and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells (HAOEC). ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .

    Journal: Scientific Reports

    Article Title: VE-cadherin shedding in vitro and in patients with aortic aneurysm and dissection

    doi: 10.1038/s41598-024-77940-3

    Figure Lengend Snippet: TNF-α and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells (HAOEC). ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .

    Article Snippet: Viability of HAOEC upon treatment with various TNF-α concentrations ranging from 100 to 1000 ng/ml was performed by an commercially available “MTT” assay (Promega, G4000, including Solubilization Solution/Stop Mix G401A and Dye Solution G402A) as described by Guo et al. .

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Diffusion-based Assay